Reducing enteric methane production from buffalo (Bubalus bubalis) by garlic oil supplementation in in vitro rumen fermentation system

Abstract Enteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock in Asian countries, contributing milk, meat and draft power, contribution enteric environmental pollution attracts attention. The present study investigated efficacy garlic ( Allium sativum ) oil reducing from Bubalus bubalis by vitro rumen fermentation. Garlic (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 (GOL-2) 166.66 (GOL-3) per litre buffered fluid] 100-ml graduated glass syringes incubated 39℃ for 24 h fermentation study. Supplementation GOL-1 increased p < 0.05) total comparison with GOL-3; however, it remained comparable > control GOL-2. Graded doses inclusions reduced 0.001) concentration (%) (ml/g DM), irrespective concentrations. feed degradability, volatile fatty acids microbial biomass (MBP) were not affected GOL-1, but these tended decrease GOL-2 marked reduction 0.01) GOL-3. NH 3 –N fluid presence oil, concentration, suggests deamination inhibiting proteolytic bacterial population. activities ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) lower dose (GOL-1) oil; activity liquor evident higher (GOL-2 GOL-3) supplementation. This shows positive impact supplementation low (33.33 µl/l fluid) production, thereby, abatement without affecting digestibility.